Journal: BMC Microbiology

Article Title: Synergistic potential of Leu 10 -teixobactin and cefepime against multidrug-resistant Staphylococcus aureus

doi: 10.1186/s12866-024-03577-x

Figure Lengend Snippet: Standard inoculum time-kill graphs (Initial inoculum/strain log 10 CFU/mL ~ 6.0) of Leu 10 -teixobactin (L 10 TXB) and cefepime (CEF) alone and in combination against S. aureus ATCC™29213, ATCC™43300, ATCC™700698, ATCC™700699, and JKD6159. Bacterial counts are presented as the mean from two independent repeats

Article Snippet: Additive antibacterial activity was observed in combination-treated ATCC™700699 (> 1.1-log 10 CFU decrease or difference in the colony counts relative to L 10 TXB monotherapy at 6 h) and ATCC™700698 bacteria (-1.2Δlog 10 CFU compared to the initial inoculum at 24 h), likely in part, due to having thicker cell walls [ , ].

Techniques:

Journal: BMC Microbiology

Article Title: Synergistic potential of Leu 10 -teixobactin and cefepime against multidrug-resistant Staphylococcus aureus

doi: 10.1186/s12866-024-03577-x

Figure Lengend Snippet: Real-time quantitative PCR analysis of icaA , atlA , sarA , mecA , and pbp2 gene transcription in log-phase ATCC™43300 at 4 h post-treatment with Leu 10 -teixobactin and cefepime, alone and in combination. Gene expression levels were normalized to the 16S rRNA gene. The asterisks represent the statistical difference between the treatment groups and the untreated control, determined by one-way analysis of variance (ANOVA) followed by Tukey’s honest significant test. Significance was accepted when the p -value < 0.05* (**** p -value < 0.0001)

Article Snippet: Additive antibacterial activity was observed in combination-treated ATCC™700699 (> 1.1-log 10 CFU decrease or difference in the colony counts relative to L 10 TXB monotherapy at 6 h) and ATCC™700698 bacteria (-1.2Δlog 10 CFU compared to the initial inoculum at 24 h), likely in part, due to having thicker cell walls [ , ].

Techniques: Real-time Polymerase Chain Reaction, Expressing, Control

Journal: Scientific reports

Article Title: Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections.

doi: 10.1038/s41598-023-35364-5

Figure Lengend Snippet: Figure 2. Scanning electron micrographs depicting fibers at 2500× and 5000× magnification of EBHT fibers (a, b), JG004 fibers (c, d), and placebo fibers (e, f). Box-whisker plots showing the fiber diameter distribution of bacteriophage-loaded and placebo fibers (g). Transmission electron micrographs of EBHT fibers (h, 43,000×), JG004 fibers (i, 9300), and placebo fibers (j, 9300×). Arrows indicate the core layer (h, j).

Article Snippet: Staphylococcus Phage EBHT (DSM 26856), Pseudomonas Phage JG004 (DSM 19871), Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 19880), and Staphylococcus aureus Rosenbach 1884 (DSM 104437) were kindly provided by Dr. Christine Rohde from the Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques: Whisker Assay, Transmission Assay

Journal: Scientific reports

Article Title: Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections.

doi: 10.1038/s41598-023-35364-5

Figure Lengend Snippet: Figure 3. Raman false-color images of placebo fibers (a), EBHT fibers (b), JG004 fibers (c), and the corresponding Raman fiber spectra (d).

Article Snippet: Staphylococcus Phage EBHT (DSM 26856), Pseudomonas Phage JG004 (DSM 19871), Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 19880), and Staphylococcus aureus Rosenbach 1884 (DSM 104437) were kindly provided by Dr. Christine Rohde from the Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques:

Journal: Scientific reports

Article Title: Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections.

doi: 10.1038/s41598-023-35364-5

Figure Lengend Snippet: Figure 7. Inhibition zone diameters of EBHT (a) and JG004 fiber samples (b) against S. aureus and P. aeruginosa, respectively. Representative photographs of agar plates incubated with either bacteriophage fibers (c, d) or placebo fibers (e, f). Scale bars represent 5 cm with 1 cm intervals.

Article Snippet: Staphylococcus Phage EBHT (DSM 26856), Pseudomonas Phage JG004 (DSM 19871), Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 19880), and Staphylococcus aureus Rosenbach 1884 (DSM 104437) were kindly provided by Dr. Christine Rohde from the Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques: Inhibition, Incubation

Journal: Scientific reports

Article Title: Bacteriophage-loaded functional nanofibers for treatment of P. aeruginosa and S. aureus wound infections.

doi: 10.1038/s41598-023-35364-5

Figure Lengend Snippet: Figure 8. Zone of inhibition diameters of EBHT fibers (a) and JG004 fibers (b) directly after electrospinning (fresh) and after 1,2, and 4 weeks of storage at either ambient temperature (AT, red), 5 °C (green), or − 20 °C (blue).

Article Snippet: Staphylococcus Phage EBHT (DSM 26856), Pseudomonas Phage JG004 (DSM 19871), Pseudomonas aeruginosa (Schroeter 1872) Migula 1900 (DSM 19880), and Staphylococcus aureus Rosenbach 1884 (DSM 104437) were kindly provided by Dr. Christine Rohde from the Leibniz Institute DSMZ—German Collection of Microorganisms and Cell Cultures GmbH (Braunschweig, Germany).

Techniques: Inhibition

Journal: Italian Journal of Food Safety

Article Title: Selection of commercial protective cultures to be added in Sardinian fermented sausage to control Listeria monocytogenes

doi: 10.4081/ijfs.2022.10368

Figure Lengend Snippet: L. monocytogene s , LAB, micrococci, Coagulase Negative Staphylococci and Enterobacteriaceae mean values (log 10 CFU/g; x ®± S.D.) in SFS samples during the challenge test.

Article Snippet: For the enumeration of micrococci, Coagulase- Positive Staphylococci (CPS) and Coagulase-Negative Staphylococci (CNS), Mannitol Salt Agar (MSA, Biolife, Milan, Italy) was used.

Techniques:

Journal: Italian Journal of Food Safety

Article Title: Selection of commercial protective cultures to be added in Sardinian fermented sausage to control Listeria monocytogenes

doi: 10.4081/ijfs.2022.10368

Figure Lengend Snippet: L. monocytogenes , LAB, micrococci, Coagulase Negative Staphylococci and Enterobacteriaceae mean values log 10 CFU/g; x ® ± S.D. (positive samples/total) in SFS samples produced without and with the addition of protective culture A.

Article Snippet: For the enumeration of micrococci, Coagulase- Positive Staphylococci (CPS) and Coagulase-Negative Staphylococci (CNS), Mannitol Salt Agar (MSA, Biolife, Milan, Italy) was used.

Techniques: Produced